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Title   두경부 편평상피세포암 세포주들에서 레틴산에 의한 세포성장억제기전에 관한연구 ( The Mechanism of Retinoic Acid - induced Growth Suppression in Head and Neck Squamous Cancer Cell Lines )
Publicationinfo   2000 Jan; 032(04): 783-793.
Key_word   Retinoic acid, Apoptosis, Cell cycle arrest
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Abstract   Purpose: Retinonic acid (RA) has been reported to induce differentiation and growth inhibition in various head and neck squamous cancer cell (HNSCC) lines. We hypothesized that this growth inhibition might be explained by RA-induced apoptosis on cell cycle arrest mechanism. Therefore, we studied the degree of RA-induced apoptosis with variable RA concentration and exposure duration. Material and Methods: The flow cytometric evaluation of apoptosis degree and cell cycles were carried out with 7-amino actinomycin D (7AAD) and propium iodide (Pl) respectively, with various RA exposure durations (2, 3, 6 day) and concentrations (conrol, 10 ', 10 , 10 , 10 ', 10 mole). Two different HNSCC lines (1483, SqCC/Y 1) were used and the experiment was repeated twice. Results: The maximal fraction of apoptosis in 1483 and SqCC/Yl cell lines were observed at same concentration and exposure duration (1483: 6" day & 10 ', mole, and SqCC/Yl: 6' day & 10 ' mole). In our experimental model, RA did not induce specific cell cycle arrest in these HNSCC lines. However we observed S phase fraction increase in SqCC/Yl cell line after RA treatment. Conclusion: We suppossed that in HNSCC lines, RA-induced cell growth inhibition could be explained by not only RA-induced apoptosis but also cell cycle arrest. Futher, in vitro study has been carried out to elucidate the RA-iduced cell growth inhibition mechanism in our laboratory.
저 자   백창원(Chang Won Paek),서재홍(Jae Hong Seo),최철원(Chul Won Choi),김병수(Byung Soo Kim),신상원(Sang Won Shin),김열홍(Yeul Hong Kim),김준석(Jun Suk Kim),김석진(Seok Jin Kim),김애리(A Lee kim),이갑노(Kap No Lee),김선한(Sun Han Kim),최건(Geon Choi),유영아(Young A Yoo)